Differentiation and Induction Protocols

Motor Neuron Induction and Differentiation

This protocol is an interactive and clickable pdf describing how to:

• Generate motor neural progenitor cells (MNPCs)
• Generate motor neurons from MNPCs

Dopaminergic or Cortical Neuron Differentiation

This is an interactive protocol to describe and to assist in training on how to:

• Culture dopaminergic (DA) or cortical neural progenitor cells (NPCs)
• Generate DA or cortical neurons from DA or cortical NPCs, respectively

Dopaminergic or Cortical Neural Progenitor Cell Induction

This protocol describes how to generate dopaminergic (DA) or cortical neural progenitor cells (NPCs) from induced pluripotent stem cells (iPSCs).

Generation of Forebrain Spheroids

This protocol describes how to generate forebrain spheroids from iPSCs. Forebrain spheroids are formed in stationary cultures, they contain cortical neurons and astrocytes. These spheroids can be maintained in cultures for several months allowing the study of neuronal progenitors and mature cells. 

Generation of OPCs and Oligodendrocytes from iPSCs

This protocol describes how to:

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• Generate and culture oligodendrocyte progenitor cells (OPCs) from iPSCs

• Differentiate and culture functional myelinating oligodendrocytes