2D Differentiation and Induction Protocols
Dopaminergic or Cortical Neuron Differentiation
This is an interactive protocol to describe and to assist in training on how to:
• Culture dopaminergic (DA) or cortical neural progenitor cells (NPCs)
• Generate DA or cortical neurons from DA or cortical NPCs, respectively
Dopaminergic or Cortical Neural Progenitor Cell Induction
This protocol describes how to generate dopaminergic (DA) or cortical neural progenitor cells (NPCs) from induced pluripotent stem cells (iPSCs).
Generation of OPCs and Oligodendrocytes from iPSCs
This protocol describes how to:
Generate and culture oligodendrocyte progenitor cells (OPCs) from iPSCs
Differentiate and culture functional myelinating oligodendrocytes
Cilia, iPSC-derived Neurons, and Neurodevelopmental Disorders
Watch this video to learn about the work being done at the EDDU with iPSC-derived neurons and brain organoids to understand the role of cilia in neurodevelopmental disorders.