2D Differentiation and Induction Protocols
Motor Neuron Induction and Differentiation
This protocol is an interactive and clickable pdf describing how to:
• Generate motor neural progenitor cells (MNPCs)
• Generate motor neurons from MNPCs
Dopaminergic or Cortical Neuron Differentiation
This is an interactive protocol to describe and to assist in training on how to:
• Culture dopaminergic (DA) or cortical neural progenitor cells (NPCs)
• Generate DA or cortical neurons from DA or cortical NPCs, respectively
Induction of Dopaminergic Neuronal Progenitors From iPSCs Using Microfabricated Disk
This protocol describes how to generate dopaminergic neural progenitor cells (DPCs) from induced-pluripotent stem cells (iPSCs) using microfabricated embryoid body disk device (EB disk).
This protocol describes how to:
Thaw and culture dopaminergic neural progenitor cells (DPCs).
Plate DPCs in a 96 well format to be differentiated into dopaminergic neurons (DNs)
Cilia, iPSC-derived Neurons, and Neurodevelopmental Disorders
Watch this video to learn about the work being done at the EDDU with iPSC-derived neurons and brain organoids to understand the role of cilia in neurodevelopmental disorders.