2D Differentiation and Induction Protocols

Motor Neuron Induction and Differentiation

This protocol is an interactive and clickable pdf describing how to:

• Generate motor neural progenitor cells (MNPCs)
• Generate motor neurons from MNPCs

Dopaminergic or Cortical Neuron Differentiation

This is an interactive protocol to describe and to assist in training on how to:

• Culture dopaminergic (DA) or cortical neural progenitor cells (NPCs)
• Generate DA or cortical neurons from DA or cortical NPCs, respectively

Related Publications

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Dopaminergic or Cortical Neural Progenitor Cell Induction

This protocol describes how to generate dopaminergic (DA) or cortical neural progenitor cells (NPCs) from induced pluripotent stem cells (iPSCs).

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Generation of OPCs and Oligodendrocytes from iPSCs

This protocol describes how to:

Generate and culture oligodendrocyte progenitor cells (OPCs) from iPSCs
Differentiate and culture functional myelinating oligodendrocytes

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Induction of Dopaminergic Neuronal Progenitors From iPSCs Using Microfabricated Disk

This protocol describes how to generate dopaminergic neural progenitor cells (DPCs) from induced-pluripotent stem cells (iPSCs) using microfabricated embryoid body disk device (EB disk).

This protocol describes how to:

Thaw and culture dopaminergic neural progenitor cells (DPCs).
Plate DPCs in a 96 well format to be differentiated into dopaminergic neurons (DNs)

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Cilia, iPSC-derived Neurons, and Neurodevelopmental Disorders

Watch this video to learn about the work being done at the EDDU with iPSC-derived neurons and brain organoids to understand the role of cilia in neurodevelopmental disorders.