Read about the latest updates happening at the EDDU
Release of pro-inflammatory molecules which act on OLs during MS lesion formation. Made with BioRender. Adapted from: Wu GF, Alvarez E. Neurol Clin. 2011. Levite M. J Neural Transm (Vienna). 2017. Frigo M, et al. Curr Med Chem. 2012
iPSC-Derived Oligodendrocytes - a model for regenerative potential in Multiple Sclerosis?
Congratulations to Jack Antel, Gabriela Blaszczyk,
Thomas Durcan, and Valerio Piscopo for being featured in MRM Insights for their work with iPSC-derived oligodendrocytes.
Read the full MRM article here.
Read the EDDU's monthly newsletter to learn more about the latest events and achievements happening at the EDDU!
Click here to view the April/May newsletter.
Next issue coming in July 2022!
Photo by Valerio Piscopo
EDDU Video Spotlight
Generation of homozygous PRKN, PINK1 and double PINK1/PRKN knockout cell lines from healthy induced pluripotent stem cells using CRISPR/Cas9 editing
Congratulations to Carol Chen, iPSC Colony manager at the EDDU, and to everyone else involved for their recent publication in Stem Cell Research. Click the link below to read the full article.
Auto-qPCR: A Python-based web app for automated and reproducible analysis of qPCR data
Gilles Maussion and others have developed an open-source Python software to automate processing of result spreadsheets from qPCR machines, employing calculations usually performed manually. Auto-qPCR is a tool that saves time when computing qPCR data, helping to ensure reproducibility of qPCR experiment analyses.
A streamlined CRISPR workflow to introduce mutations and generate isogenic iPSCs for modeling amyotrophic lateral sclerosis
Amyotrophic lateral sclerosis (ALS) represents a complex neurodegenerative disorder with significant genetic heterogeneity. Through a combination of iPSCs and CRISPR editing, the cells generated here will provide fundamental insights into the molecular mechanisms underlying neuron degeneration in ALS.
Rapid Generation of Ventral Spinal Cord-like Astrocytes from Human iPSCs for Modeling Non-Cell Autonomous Mechanisms of Lower Motor Neuron Disease
We are excited to announce that the article by Vincent Soubannier of the EDDU and others,"Rapid Generation of Ventral
Spinal Cord-like Astrocytes from Human iPSCs for Modeling Non-Cell Autonomous
Mechanisms of Lower Motor Neuron Disease", has been published in Cells as part
of the Special Issue Cell-Based Models of Diseases for Drug Discovery.
Midbrain organoids with an SNCA gene triplication model key features of synucleinopathy
Here, Nguyen-Vi Mohamed and others use three-dimensional midbrain organoids, differentiated from human-induced pluripotent stem cells derived from patients carrying a triplication of the SNCA gene and from CRISPR/Cas9 corrected isogenic control iPSCs. These human midbrain organoids recapitulate key feature of α-synuclein pathology observed in the brains of patients with synucleinopathies.
MYSM1 in Epigenetic Regulation of Mammalian Hematopoiesis
With the support of the McGill Regenerative Medicine network, and in partnership with the Early Drug Discovery Unit (EDDU) of the Montreal Neurological Institute, Dr. Anastasia Nijnik and her lab members have established a set of human induced pluripotent stem cells (iPSCs), carrying MYSM1 loss of function mutations.