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Protocols

iPSC Culture

This protocol describes a CRISPR-Cas9 genome editing method to generate knockout (KO) cell lines.

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Related Publications

CRISPR

2D Differentiation and Induction

This protocol describes how to generate a gene knockout cell line through a combination of techniques that includes:

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  • CRISPR-Cas9 editing;

  • Droplet digital PCR (ddPCR) technology;

  • Single cell sorting.

3D Models and Techniques

This protocol describes how to generate a gene knockout cell line through a combination of techniques that includes:

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  • CRISPR-Cas9 editing;

  • Droplet digital PCR (ddPCR) technology;

  • Single cell sorting.

Other

This protocol describes how to generate a gene knockout cell line through a combination of techniques that includes:

​

  • CRISPR-Cas9 editing;

  • Droplet digital PCR (ddPCR) technology;

  • Single cell sorting.

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