CRISPR Protocols
Generation of knockout cell lines using CRISPR-Cas9 technology
This protocol describes a CRISPR-Cas9 genome editing method to generate knockout (KO) cell lines.
CRISPR-mediated introduction of single nucleotide changes in iPSCs
This protocol describes how to edit an individual nucleotide in the genome of human induced pluripotent stem cells (iPSCs), combining CRISPR-Cas9 editing, droplet digital PCR (ddPCR) technology and antibiotic-free selection.
Generation of knockout cell lines using CRISPR-Cas9 and ddPCR technology
This protocol describes how to generate a gene knockout cell line through a combination of techniques that includes:
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CRISPR-Cas9 editing;
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Droplet digital PCR (ddPCR) technology;
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Single cell sorting.