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CRISPR Protocols
Generation of knockout cell lines using CRISPR-Cas9 technology
This protocol describes a CRISPR-Cas9 genome editing method to generate knockout (KO) cell lines.
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CRISPR-mediated introduction of single nucleotide changes in iPSCs
This protocol describes how to edit an individual nucleotide in the genome of human induced pluripotent stem cells (iPSCs), combining CRISPR-Cas9 editing, droplet digital PCR (ddPCR) technology and antibiotic-free selection.
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