CRISPR Protocols

Generation of knockout cell lines using CRISPR-Cas9 technology

This protocol describes a CRISPR-Cas9 genome editing method to generate knockout (KO) cell lines.

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CRISPR-mediated introduction of single nucleotide changes in iPSCs

This protocol describes how to edit an individual nucleotide in the genome of human induced pluripotent stem cells (iPSCs), combining CRISPR-Cas9 editing, droplet digital PCR (ddPCR) technology and antibiotic-free selection.

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Generation of knockout cell lines using CRISPR-Cas9 and ddPCR technology

This protocol describes how to generate a gene knockout cell line through a combination of techniques that includes:

  • CRISPR-Cas9 editing;

  • Droplet digital PCR (ddPCR) technology;

  • Single cell sorting.