CRISPR-mediated introduction of single nucleotide changes in iPSCs
This protocol describes how to edit an individual nucleotide in the genome of human induced pluripotent stem cells (iPSCs), combining CRISPR-Cas9 editing, droplet digital PCR (ddPCR) technology and antibiotic-free selection.
Generation of knockout cell lines using CRISPR-Cas9 and ddPCR technology
This protocol describes how to generate a gene knockout cell line through a combination of techniques that includes:
Droplet digital PCR (ddPCR) technology;
Single cell sorting.