top of page

CRISPR Protocols

Generation of knockout cell lines using CRISPR-Cas9 technology

This protocol describes a CRISPR-Cas9 genome editing method to generate knockout (KO) cell lines.

​

Related Publications

CRISPR-mediated introduction of single nucleotide changes in iPSCs

This protocol describes how to edit an individual nucleotide in the genome of human induced pluripotent stem cells (iPSCs), combining CRISPR-Cas9 editing, droplet digital PCR (ddPCR) technology and antibiotic-free selection.

​

Related Publications

Generation of knockout cell lines using CRISPR-Cas9 and ddPCR technology

This protocol describes how to generate a gene knockout cell line through a combination of techniques that includes:

​

  • CRISPR-Cas9 editing;

  • Droplet digital PCR (ddPCR) technology;

  • Single cell sorting.

Neuro logo
McGill Logo
muhc logo
killam logo

Copyright © 2021 The Neuro's EDDU. All rights reserved.

bottom of page