Miscellaneous Protocols

How to analyze your sequencing data from plasmids

In this protocol, we describe our method used for analyzing sequence data obtained with our Applied Biosystems SeqStudio Genetic Analyzer instrument and analyzed using SnapGene software.

SeqStudio DNA Sequencing

This protocol describes how to:

  • Perform a cycle sequencing of DNA using the BigDye™ Terminator v3.1 Cycle Sequencing Kit

  • Analyse a sequencing reaction using the Applied Biosystems SeqStudio™ Genetic Analyzer

Quality Control Characterization of α-Synuclein Preformed Fibrils (PFFs)

This protocol describes how to:

• Determine fibril length of sonicated unlabelled α-synuclein preformed fibrils (PFFs) by dynamic light scattering (DLS) and electron microscopy (EM)
• Confirm cellular uptake of fluorescently-labelled α-synuclein PFFs by immunofluorescence (IF)

Related Publications

Uptake of α-Synuclein preformed fibrils (PFFs) in dopaminergic neural progenitor cells

This protocol describes an assay to quantify the uptake of α-synuclein aggregates by neuronal cells by monitoring the uptake of fluorescently labelled α-synuclein preformed fibrils (PFFs) in dopaminergic (DA) neural progenitor cells (NPCs) derived from induced pluripotent stem cells (iPSCs).

Production of Recombinant α-Synuclein Monomers and Preformed Fibrils (PFFs)

This protocol describes how to:
• Produce and purify recombinant human and mouse α-synuclein in monomeric form from transformed bacteria
• Generate α-synuclein preformed fibrils (PFFs) from recombinant monomeric α-synuclein
• Fluorescently label α-synuclein PFFs

Related Publications

Management of High Content Screening Data

The purpose of this document is to outline the workflow from data generation to data storage and analysis using the ThermoFisher Scientific CellInsight CX7 High Content Screening (HCS) Platform in fluorescence-based cellular assays. It provides guidelines on image acquisition, image analysis and data management.

FlowJo Training with Julien Sirois

Watch this video to learn how to use the FlowJo software for flow cytometry data analysis.